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Cannot open database \"mydb\" requested by the login. The login failed.\r\nLogin failed for user 'IIS APPPOOL\\ASP.NET v4.0'

Error:
Cannot open database \"MYDB\" requested by the login. The login failed.\r\nLogin failed for user 'IIS APPPOOL\\ASP.NET v4.0'."}

Try:
Note 'IIS APPPool' in the error - it is looking for 'NETWORK SERVICE' to be set as identity.

To do this,
Go to : IIS Manager  > Application Pools > Select - Your Application Pool (That you have mapped to your application , like ASP.NET v4.0)
On the RHS > Click 'Advanced Settings' > Look For 'Process ' > 'identity' > From the dropdown Select 'NETWORK SERVICE'  > click OK.
Now try running your application.

Comments

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